
Singlemoleculedetection(SMD)andanalysisisanelegantwaytoexaminedynamicsandinteractionsinsidecellularsystems.ThenewLeicaTCSSMDSeriesperfectlyintegratestheconfocalmicroscopeLeicaTCSSP8andtheSMDspecificsoftwareandhardwarefromPicoQuantintoonesystem.
ThewellprovencomponentsconstituteaflexIBLeplatformforaplethoraoftimeresolvedtechniquesincludingFCS,FCCS,FLIM andFLCS.Theglobalsystemcontrolanditselegantinterfaceopenthedoortoamultitudeofbiophysicaltechniqueswhichprovidequantitativeparametersformodelbuilding.

Globalexperimentcontrolbyfullsystemintegration
Withthefullintegrationofallcomponentstheusercancontrolthecompleteexperimentviaoneinterface.Dedicatedapplicationwizardsprovidereproducibleresultsquicklyandeasily.Thehighdegreeofautomationallowsnewrecordingstrategies.OnlineSMDdatadisplayandinteractivefeedbackgrantsatightcontrolofdataquality.

Spectralandlifetimeinformationatonce–Combinethepower!
ThecombinationofaLeicaTCSSP8XwhitelightlasersystemwithspectralFLIMdetectionresultsinatuneable,filterfreeFLIMsystem.HighestFLIMdataqualityisachievedbytuningboth,excitationanddetectiontotheoptimalspectralrange.UsingtheconvenienceoftheSMDFLIMwizard,automatedFLIMexcitationanddetectionstackscanbeacquired,whichisveryusefultoseparatefluorescenceofmultiplestainedsamplesandtocharacterizenewdyes.

QuickResults-betterdatareliABIlity
TheonlinedisplayofimportantstatisticalcontrolparametersuchasthecorrelationcurveandfastFLIMimage,thecountrate,themolecularbrightnessorthemaximalphotoncountnumberallowthecontinuousevaluationofthedataqualityduringtherunningFCSorFLIMexperiment.SMDdatacanbealreadyinterpretedduringthemeasurementseries.

MaximumflexibilityinSMDanalysis
DuetoauniversalSMDrawdataformattheverysamedatafilecanbeanalyzedindifferentways.Dependingonhardwareandexperimentaldesign,bothcorrelationandlifetimeinformationcanbeextractedfromasinglemeasurementyieldingamaximumofinformationaboutthesample.FCSsystemscanbeupgradedwithFLIMandviceversatoachievethemostflexibleFLCSconfiguration.